The Prevalence of Carbon-13 in Respiratory Carbon Dioxide As an Indicator of the Type of Endogenous Substrate. The change from lipid to carbohydrate during the respiratory rise in potato slices

Isotope discrimination is a common feature of biosynthesis in nature, with the result that different classes of carbon compounds frequently display different 13C/12C ratios. The 13C/12C ratio of lipid in potato tuber tissue is considerably lower than that for starch or protein. We have collected respiratory CO2 from potato discs in successive periods through 24 hr from the time of cutting—an interval in which the respiration rate rises 3–5-fold. The 13C/12C ratio of the evolved CO2 was determined for each period, and compared with the 13C/12C ratios of the major tissue metabolites. In the first hours the carbon isotope ratio of the CO2 matches that of lipid. With time, the ratio approaches that typical of starch or protein. An estimation has been made of the contribution of lipid and carbohydrate to the total respiration at each juncture. In connection with additional observations, it was deduced that the basal, or initial, respiration represents lipid metabolism —- possibly the alpha-oxidation of long chain fatty acids -— while the developed repiration represents conventional tricarboxylic acid cycle oxidation of the products of carbohydrate glycolysis. The true isotopic composition of the respiratory CO2 may be obscured by fractionation attending the refixation of CO2 during respiration, and by CO2 arising from dissolved CO2 and bicarbonate preexisting in the tuber. Means are described for coping with both pitfalls

Translational Retinal Research and Therapies.

The following review summarizes the state of the art in representative aspects of gene therapy/translational medicine and evolves from a symposium held at the School of Veterinary Medicine, University of Pennsylvania on November 16, 2017 honoring Dr. Gustavo Aguirre, recipient of ARVO's 2017 Proctor Medal. Focusing on the retina, speakers highlighted current work on moving therapies for inherited retinal degenerative diseases from the laboratory bench to the clinic

Three-geometry and reformulation of the Wheeler-DeWitt equation

A reformulation of the Wheeler-DeWitt equation which highlights the role of gauge-invariant three-geometry elements is presented. It is noted that the classical super-Hamiltonian of four-dimensional gravity as simplified by Ashtekar through the use of gauge potential and densitized triad variables can furthermore be succinctly expressed as a vanishing Poisson bracket involving three-geometry elements. This is discussed in the general setting of the Barbero extension of the theory with arbitrary non-vanishing value of the Immirzi parameter, and when a cosmological constant is also present. A proposed quantum constraint of density weight two which is polynomial in the basic conjugate variables is also demonstrated to correspond to a precise simple ordering of the operators, and may thus help to resolve the factor ordering ambiguity in the extrapolation from classical to quantum gravity. Alternative expression of a density weight one quantum constraint which may be more useful in the spin network context is also discussed, but this constraint is non-polynomial and is not motivated by factor ordering. The article also highlights the fact that while the volume operator has become a preeminient object in the current manifestation of loop quantum gravity, the volume element and the Chern-Simons functional can be of equal significance, and need not be mutually exclusive. Both these fundamental objects appear explicitly in the reformulation of the Wheeler-DeWitt constraint.Comment: 10 pages, LaTeX fil

Reality Conditions and Ashtekar Variables: a Different Perspective

We give in this paper a modified self-dual action that leads to the $SO(3)$-ADM formalism without having to face the difficult second class constraints present in other approaches (for example if one starts from the Hilbert-Palatini action). We use the new action principle to gain some new insights into the problem of the reality conditions that must be imposed in order to get real formulations from complex general relativity. We derive also a real formulation for Lorentzian general relativity in the Ashtekar phase space by using the modified action presented in the paper.Comment: 22 pages, LATEX, Preprint CGPG-94/10-

Is Barbero's Hamiltonian formulation a Gauge Theory of Lorentzian Gravity?

This letter is a critique of Barbero's constrained Hamiltonian formulation of General Relativity on which current work in Loop Quantum Gravity is based. While we do not dispute the correctness of Barbero's formulation of general relativity, we offer some criticisms of an aesthetic nature. We point out that unlike Ashtekar's complex SU(2) connection, Barbero's real SO(3) connection does not admit an interpretation as a space-time gauge field. We show that if one tries to interpret Barbero's real SO(3) connection as a space-time gauge field, the theory is not diffeomorphism invariant. We conclude that Barbero's formulation is not a gauge theory of gravity in the sense that Ashtekar's Hamiltonian formulation is. The advantages of Barbero's real connection formulation have been bought at the price of giving up the description of gravity as a gauge field.Comment: 12 pages, no figures, revised in the light of referee's comments, accepted for publication in Classical and Quantum Gravit

The reality conditions for the new canonical variables of General Relativity

We examine the constraints and the reality conditions that have to be imposed in the canonical theory of 4--d gravity formulated in terms of Ashtekar variables. We find that the polynomial reality conditions are consistent with the constraints, and make the theory equivalent to Einstein's, as long as the inverse metric is not degenerate; when it is degenerate, reality conditions cannot be consistently imposed in general, and the theory describes complex general relativity.Comment: 11

General covariance, and supersymmetry without supersymmetry

An unusual four-dimensional generally covariant and supersymmetric SU(2) gauge theory is described. The theory has propagating degrees of freedom, and is invariant under a local (left-handed) chiral supersymmetry, which is half the supersymmetry of supergravity. The Hamiltonian 3+1 decomposition of the theory reveals the remarkable feature that the local supersymmetry is a consequence of Yang-Mills symmetry, in a manner reminiscent of how general coordinate invariance in Chern-Simons theory is a consequence of Yang-Mills symmetry. It is possible to write down an infinite number of conserved currents, which strongly suggests that the theory is classically integrable. A possible scheme for non-perturbative quantization is outlined. This utilizes ideas that have been developed and applied recently to the problem of quantizing gravity.Comment: 17 pages, RevTeX, two minor errors correcte

The Husain-Kuchar Model: Time Variables and Non-degenerate Metrics

We study the Husain-Kuchar model by introducing a new action principle similar to the self-dual action used in the Ashtekar variables approach to Quantum Gravity. This new action has several interesting features; among them, the presence of a scalar time variable that allows the definition of geometric observables without adding new degrees of freedom, the appearance of a natural non-degenerate four-metric and the possibility of coupling ordinary matter.Comment: LaTeX, 22 pages, accepted for publication in Phys. Rev.

BF Actions for the Husain-Kuchar Model

We show that the Husain-Kuchar model can be described in the framework of BF theories. This is a first step towards its quantization by standard perturbative QFT techniques or the spin-foam formalism introduced in the space-time description of General Relativity and other diff-invariant theories. The actions that we will consider are similar to the ones describing the BF-Yang-Mills model and some mass generating mechanisms for gauge fields. We will also discuss the role of diffeomorphisms in the new formulations that we propose.Comment: 21 pages (in DIN A4 format), minor typos corrected; to appear in Phys. Rev.

Reply to Townes-Anderson: RPE65 Gene Therapy Does Not Alter the Natural History of Retinal Degeneration

We appreciate the interest shown by TownesAnderson in our article examining the natural history of retinal degeneration in Leber congenital amaurosis caused by retinal pigment epithelium-specific protein 65kDa (RPE65) mutations and evaluating the consequences of gene augmentation therapy. Townes-Anderson’s remarks focused on the final phrase of the last sentence of the Discussion of our article. In the full sentence, we suggested that in the future, agents to reduce cell death could be delivered in combination with a more advanced version of the gene augmentation therapy that reaches not only remaining rods and extrafoveal cones but also foveal cone photoreceptors